ACE2, also known as angiotensin-converting enzyme 2, is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE. ACE2 cleaves angiotensin 1 to angiotensin 1-9 and angiotensin 2 to angiotensin 1-7 as a carboxypeptidase. It may be an important regulator of heart function and have a protective role in acute lung injury. Partial recombinant protein corresponding to Gln18-Ser740 from human ACE2, fused to hFc-Tag at C-terminal, expressed in Mammalian cells. Molecular Weight: ~111.1kD Biological Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2ug/ml can bind human ACE2, the EC50 of human ACE2 protein is 56.64-103.6ng/ml 2. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5ug/ml can bind human ACE2, the EC50 of human ACE2 protein is 31.80-44.69ng/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5ug/ml can bind human ACE2, the EC50 of human ACE2 protein is 115.0-274.9ng/ml. 4. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2ug/ml can bind SARS-CoV-2-S1-RBD, the EC50 of human ACE2 protein is 8.363-12.82ng/ml. 5. SARS-CoV-2 Spike protein RBD his/myc tag captured on COOH chip can bind 544434 with an affinity constant of 13.8pM as detected by LSPR Assay. AA Sequence: QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS Storage and Stability: Lyophilized and reconstituted products may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
