Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases. This resin works well with relatively easy to separate His-tagged proteins. High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). Bead Geometry & Size: Spherical Standard: ~50-150um Crosslinked: Yes Agarose %: 6% Matrix: Stable in all commonly used reagents Binding/Loading Capacity (umol Me2+/ml gel): 20-40 Antimicrobial Agent: 20% Ethanol Storage Temperature: 2-8C
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