The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 993bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCAGCCCC AGTCCGTTCT GCACAGCGGC TACTTCCACC CACTACTTCG GGCCTGGCAG ACAGCCACCA CCACCCTCAA TGCCTCCAAC CTCATCTACC CCATCTTTGT CACGGATGTT CCTGATGACA TACAGCCTAT CACCAGCCTC CCAGGAGTGG CCAGGTATGG TGTGAAGCGG CTGGAAGAGA TGCTGAGGCC CTTGGTGGAA GAGGGCCTAC GCTGTGTCTT GATCTTTGGC GTCCCCAGCA GAGTTCCCAA GGACGAGCGG GGTTCCGCAG CTGACTCCGA GGAGTCCCCA GCTATTGAGG CAATCCATCT GTTGAGGAAG ACCTTCCCCA ACCTCCTGGT GGCCTGTGAT GTCTGCCTGT GTCCCTACAC CTCCCATGGT CACTGCGGGC TCCTGAGTGA AAACGGAGCA TTCCGGGCTG AGGAGAGCCG CCAGCGGCTG GCTGAGGTGG CATTGGCGTA TGCCAAGGCA GGATGTCAGG TGGTAGCCCC GTCGGACATG ATGGATGGAC GCGTGGAAGC CATCAAAGAG GCCCTGATGG CACATGGACT TGGCAACAGG GTATCGGTGA TGAGCTACAG TGCCAAATTT GCTTCCTGTT TCTATGGCCC TTTCCGGGAT GCAGCTAAGT CAAGCCCAGC TTTTGGGGAC CGCCGCTGCT ACCAGCTGCC CCCTGGAGCA CGAGGCCTGG CTCTCCGAGC TGTGGACCGG GATGTACGGG AAGGAGCTGA CATGCTCATG GTGAAGCCGG GAATGCCCTA CCTGGACATC GTGCGGGAGG TAAAGGACAA GCACCCTGAC CTCCCTCTCG CCGTGTACCA CGTCTCTGGA GAGTTTGCCA TGCTGTGGCA TGGAGCCCAG GCCGGGGCAT TTGATCTCAA GGCTGCCGTA CTGGAGGCCA TGACTGCCTT CCGCAGAGCA GGTGCTGACA TCATCATCAC CTACTACACA CCGCAGCTGC TGCAGTGGCT GAAGGAGGAA TGA Translation Sequence: MQPQSVLHSG YFHPLLRAWQ TATTTLNASN LIYPIFVTDV PDDIQPITSL PGVARYGVKR LEEMLRPLVE EGLRCVLIFG VPSRVPKDER GSAADSEESP AIEAIHLLRK TFPNLLVACD VCLCPYTSHG HCGLLSENGA FRAEESRQRL AEVALAYAKA GCQVVAPSDM MDGRVEAIKE ALMAHGLGNR VSVMSYSAKF ASCFYGPFRD AAKSSPAFGD RRCYQLPPGA RGLALRAVDR DVREGADMLM VKPGMPYLDI VREVKDKHPD LPLAVYHVSG EFAMLWHGAQ AGAFDLKAAV LEAMTAFRRA GADIIITYYT PQLLQWLKEE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
