AP1AR is necessary for adaptor protein complex 1 (AP-1) -dependent transport between the trans-Golgi network and endosomes. It regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 909bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGGGAACTGCTGCTGGACGCAGTGCTTCGGACTGCTTCGCAAGGAAGCGGGGCGGCTGCAGCGAGTAGGCGGCGGCGGAGGATCCAAGTATTTTAGAACATGCTCAAGAGGTGAGCACTTAACAATAGAGTTTGAGAATCTAGTAGAAAGTGATGAAGGGGAGAGCCCAGGAAGCAGTCATAGGCCTCTTACTGAGGAAGAAATTGTTGACCTAAGAGAAAGGCATTATGATTCCATTGCCGAAAAACAAAAAGATCTTGATAAGAAAATTCAAAAAGAGTTAGCCTTACAAGAAGAGAAGTTAAGACTAGAAGAAGAAGCTTTATACGCTGCACAGCGTGAAGCAGCCAGGGCAGCAAAGCAGCGAAAGCTCTTGGAGCAAGAAAGGCAGAGAATTGTGCAGCAATATCATCCTTCCAACAATGGAGAATATCAAAGTTCAGGACCAGAAGATGACTTCGAATCTTGTTTGAGAAATATGAAGTCACAGTATGAAGTTTTTCGAAGTAGTAGACTCTCATCAGATGCTACAGTTTTGACACCAAATACAGAAAGCAGTTGTGATTTAATGACCAAAACTAAATCAACTAGTGGAAATGACGACAGCACATCCTTAGATCTAGAGTGGGAAGATGAAGAAGGAATGAATAGAATGCTTCCAATGAGAGAACGTTCCAAAACAGAGGAAGACATTCTACGGGCAGCACTTAAGTATAGCAACAAGAAGACTGGAAGTAATCCTACATCAGCCTCTGATGATTCCAATGGGCTGGAGTGGGAAAATGATTTTGTTAGTGCCGAAATGGATGATAATGGAAATTCCGAGTATTCTGGATTTGTAAATCCTGTATTAGAACTGTCTGATTCTGGCATAAGGCATTCTGACACAGATCAACAGACTCGATAG Translation Sequence: MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE SDEGESPGSSHRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE KLRLEEEALY AAQREAARAAKQRKLLEQER QRIVQQYHPS NNGEYQSSGP EDDFESCLRN MKSQYEVFRS SRLSSDATVLTPNTESSCDL MTKTKSTSGN DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYSNKKTGSNPTS ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQTR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
