CAPZA3 encodes an actin capping protein, one of the F-actin capping protein alpha subunit family. It is predominantly localized to the neck region of ejaculated sperm, other immunohistochemical signals were found in the tail and postacrosomal regions. CAPZA3 may also form heterodimers of alpha and beta subunits. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 900bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGACACTTAGCGTGCTGAGCAGGAAGGACAAGGAAAGAGTAATTCGCAGACTGTTATTACAGGCCCCTCCAGGGGAATTTGTAAATGCCTTTGATGATCTCTGTCTGCTTATCCGTGATGAAAAACTTATGCACCACCAAGGTGAGTGTGCAGGCCACCAACACTGCCAAAAATATTCTGTACCACTCTGCATCGATGGAAATCCAGTACTCTTGTCTCACCACAATGTAATGGGCGACTACCGATTTTTTGACCATCAAAGCAAACTTTCTTTCAAATATGACCTGCTTCAAAATCAGCTGAAAGACATCCAAAGTCATGGTATCATTCAGAATGAGGCAGAATACCTGAGAGTTGTTCTTCTGTGCGCCTTAAAACTGTATGTGAATGACCACTATCCAAAAGGAAATTGCAACATGCTGAGAAAAACTGTCAAAAGTAAGGAGTACTTGATAGCTTGCATTGAAGATCACAACTATGAAACAGGAGAGTGCTGGAACGGACTTTGGAAATCTAAATGGATTTTCCAAGTTAACCCATTTCTAACCCAAGTAACGGGAAGAATATTTGTGCAAGCTCACTTCTTCAGGTGTGTCAACCTTCATATTGAAATATCCAAGGACCTGAAAGAAAGCTTGGAAATAGTTAACCAAGCTCAACTGGCTCTAAGTTTTGCAAGGCTTGTGGAAGAGCAAGAGAACAAATTTCAAGCTGCAGTCTTGGAAGAATTACAGGAGTTATCCAATGAAGCCCTGAGAAAAATTCTACGAAGGGATCTTCCAGTGACCCGCACTCTTATTGACTGGCACAGGATACTCTCTGACTTGAATCTGGTGATGTATCCTAAATTAGGATATGTCATTTATTCAAGAAGTGTGTTGTGCAACTGGATAATATAA Translation Sequence: MTLSVLSRKD KERVIRRLLL QAPPGEFVNA FDDLCLLIRD EKLMHHQGEC AGHQHCQKYSVPLCIDGNPV LLSHHNVMGD YRFFDHQSKL SFKYDLLQNQ LKDIQSHGII QNEAEYLRVVLLCALKLYVN DHYPKGNCNM LRKTVKSKEY LIACIEDHNY ETGECWNGLW KSKWIFQVNPFLTQVTGRIF VQAHFFRCVN LHIEISKDLK ESLEIVNQAQ LALSFARLVE EQENKFQAAVLEELQELSNE ALRKILRRDL PVTRTLIDWH RILSDLNLVM YPKLGYVIYS RSVLCNWII Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
