Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2, 4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2, 4-dienoyl-CoAs, but also towards 2, 4, 7, 10, 13, 16, 19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 879bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCCCAGCCGCCGCCCGACGTGGAGGGGGACGACTGTCTCCCCGCGTACCGCCACCTCTTCTGCCCGGACCTGCTGCGGGACAAAGTGGCCTTCATCACAGGAGGCGGCTCTGGGATTGGGTTCCGGATTGCTGAGATTTTCATGCGGCACGGCTGCCATACGGTGATTGCCAGTAGGAGCCTGCCGCGAGTGCTGACGGCCGCCAGGAAGCTGGCTGGGGCCACCGGCCGGCGCTGCCTCCCTCTCTCTATGGACGTCCGAGCGCCCCCAGCTGTCATGGCCGCCGTGGACCAGGCTCTGAAGGAGTTTGGCAGAATCGACATTCTCATTAACTGTGCGGCCGGGAACTTCCTGTGCCCCGCTGGCGCCTTGTCCTTCAACGCCTTCAAGACCGTGATGGACATCGATACCAGCGGCACCTTCAATGTGTCTCGTGTGCTCTATGAGAAGTTCTTCCGGGACCACGGAGGGGTGATCGTGAACATCACTGCCACCCTGGGGAACCGGGGGCAGGCGCTCCAGGTGCATGCAGGCTCCGCCAAGGCCGCTGTGGACGCGATGACGCGGCACTTGGCTGTGGAGTGGGGTCCCCAAAACATCCGCGTCAACAGCCTCGCCCCTGGCCCCATCAGTGGCACAGAGGGGCTCCGGCGACTGGGTGGCCCTCAGGCCAGCCTGAGCACCAAGGTCACTGCCAGCCCGCTGCAGAGGCTGGGGAACAAGACCGAGATCGCCCACAGCGTGCTCTACCTGGCCAGCCCTCTGGCTTCCTACGTGACGGGGGCCGTGCTGGTGGCCGATGGCGGGGCATGGTTGACGTTCCCAAACGGTGTCAAAGGGCTGCCGGATTTCGCATCCTTCTCTGCTAAGCTCTAG Translation Sequence: MAQPPPDVEG DDCLPAYRHL FCPDLLRDKV AFITGGGSGI GFRIAEIFMR HGCHTVIASRSLPRVLTAAR KLAGATGRRC LPLSMDVRAP PAVMAAVDQA LKEFGRIDIL INCAAGNFLCPAGALSFNAF KTVMDIDTSG TFNVSRVLYE KFFRDHGGVI VNITATLGNR GQALQVHAGSAKAAVDAMTR HLAVEWGPQN IRVNSLAPGP ISGTEGLRRL GGPQASLSTK VTASPLQRLGNKTEIAHSVL YLASPLASYV TGAVLVADGG AWLTFPNGVK GLPDFASFSA KL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
