DDGCR6L is a protein that shares homology with the Drosophila gonadal protein, expressed in gonadal tissues and germ cells, and with the human laminin gamma-1 chain that functions in cell attachment and migration. This protein is located in a region of chromosome 22 implicated in the DiGeorge syndrome, one facet of a broader collection of anomalies referred to as the CATCH 22 syndrome. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 723bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCTGAGGAGCTGCGCCGCGCGCCTCCGCACGCTGGGGGCTCTGTGCCGGCCGCCAGTAGGCCGGCGCCTGCCGGGAAGCGAGCCGCGACCCGAGCTGAGGTCATTTTCTTCTGAGGAAGTCATTCTTAAGGACTGTTCTGTCCCCAACCCCAGCTGGAACAAGGACCTAAGACTGCTCTTTGACCAGTTTATGAAGAAATGTGAAGATGGCTCCTGGAAACGTTTGCCTTCATATAAACGTACACCTACTGAATGGATTCAAGACTTCAAAACCCATTTTCTTGACCCAAAGCTTATGAAAGAAGAACAAATGTCACAGGCCCAGCTCTTCACCAGAAGCTTTGATGATGGCCTGGGCTTTGAATACGTGATGTTCTACAATGACATTGAGAAAAGGATGGTTTGCTTATTTCAAGGAGGCCCTTACCTGGAAGGACCACCTGGATTCATTCATGGAGGTGCCATTGCAACCATGATTGATGCTACTGTTGGTATGTGTGCAATGATGGCTGGGGGAATCGTCATGACTGCCAATCTCAACATCAATTATAAAAGACCTATCCCTCTTTGTTCTGTTGTTATGATAAATAGCCAACTTGATAAAGTTGAAGGAAGGAAATTTTTTGTTTCCTGTAATGTTCAGAGTGTTGATGAGAAGACCCTATACTCAGAGGCGACAAGCTTATTTATAAAGCTGAATCCTGCTAAAAGTCTGACATAA Translation Sequence: MLRSCAARLR TLGALCRPPV GRRLPGSEPR PELRSFSSEE VILKDCSVPN PSWNKDLRLL FDQFMKKCED GSWKRLPSYK RTPTEWIQDF KTHFLDPKLM KEEQMSQAQL FTRSFDDGLG FEYVMFYNDI EKRMVCLFQG GPYLEGPPGF IHGGAIATMI DATVGMCAMM AGGIVMTANL NINYKRPIPL CSVVMINSQL DKVEGRKFFV SCNVQSVDEK TLYSEATSLF IKLNPAKSLT Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
