DNAJB9 is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70kD heat shock proteins. This gene is a member of the type 2 subgroup of DnaJ proteins. The encoded protein is localized to the endoplasmic reticulum. This protein is induced by endoplasmic reticulum stress and plays a role in protecting stressed cells from apoptosis. Vector Description: This shuttle vector contains the complete ORF. It is inseted EcoR I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 672bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCTACTC CCCAGTCAAT TTTCATCTTT GCAATCTGCA TTTTAATGAT AACAGAATTA ATTCTGGCCT CAAAAAGCTA CTATGATATC TTAGGTGTGC CAAAATCGGC ATCAGAGCGC CAAATCAAGA AGGCCTTTCA CAAGTTGGCC ATGAAGTACC ACCCTGACAA AAATAAGAGC CCGGATGCTG AAGCAAAATT CAGAGAGATT GCAGAAGCAT ATGAAACACT CTCAGATGCT AATAGACGAA AAGAGTATGA TACACTTGGA CACAGTGCTT TTACTAGTGG TAAAGGACAA AGAGGTAGTG GAAGTTCTTT TGAGCAGTCA TTTAACTTCA ATTTTGATGA CTTATTTAAA GACTTTGGCT TTTTTGGTCA AAACCAAAAC ACTGGATCCA AGAAGCGTTT TGAAAATCAT TTCCAGACAC GCCAGGATGG TGGTTCCAGT AGACAAAGGC ATCATTTCCA AGAATTTTCT TTTGGAGGTG GATTATTTGA TGACATGTTT GAAGATATGG AGAAAATGTT TTCTTTTAGT GGTTTTGACT CTACCAATCA GCATACAGTA CAGACTGAAA ATAGATTTCA TGGATCTAGC AAGCACTGCA GGACTGTCAC TCAACGAAGA GGAAATATGG TTACTACATA CACTGACTGT TCAGGACAGT AG Translation Sequence: MATPQSIFIF AICILMITEL ILASKSYYDI LGVPKSASER QIKKAFHKLA MKYHPDKNKS PDAEAKFREI AEAYETLSDA NRRKEYDTLG HSAFTSGKGQ RGSGSSFEQS FNFNFDDLFK DFGFFGQNQN TGSKKRFENH FQTRQDGGSS RQRHHFQEFS FGGGLFDDMF EDMEKMFSFS GFDSTNQHTV QTENRFHGSS KHCRTVTQRR GNMVTTYTDC SGQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
