DYNLL1, also known as DLC8 or PIN (protein inhibitor of neuronal nitric oxide synthase), has been identified as a protein that interacts with NOS1 resulting in NOS1 inhibition. Binding of this protein destabilizes NOS1 (Neuronal nitric oxide synthase) dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. DYNLL1 is a ubiquitously expressed protein that exhibits high expression in testis and moderate expression in brain. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 270bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTGCGACC GAAAGGCCGT GATCAAAAAT GCGGACATGT CGGAAGAGAT GCAACAGGAC TCGGTGGAGT GCGCTACTCA GGCGCTGGAG AAATACAACA TAGAGAAGGA CATTGCGGCT CATATCAAGA AGGAATTTGA CAAGAAGTAC AATCCCACCT GGCATTGCAT CGTGGGGAGG AACTTCGGTA GTTATGTGAC ACATGAAACC AAACACTTCA TCTACTTCTA CCTGGGCCAA GTGGCCATTC TTCTGTTCAA ATCTGGTTAA Translation Sequence: MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
