FBXL14 is a substrate-recognition component of some SCF (SKP1-CUL1-F-box protein) -type E3 ubiquitin-protein ligase complexes. The SCF (FBXL14) complex acts by mediating ubiquitination and subsequent degradation of SNAI1. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1257bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGAGACCCACATCTCATGCCTGTTCCCGGAGCTGCTGGCCATGATCTTCGGCTACCTGGACGTCCGGGACAAGGGGCGCGCGGCGCAGGTGTGCACCGCCTGGCGGGACGCCGCCTACCACAAGTCGGTGTGGCGGGGGGTGGAGGCCAAGCTGCACCTGCGCCGGGCCAACCCGTCGCTGTTCCCCAGCCTGCAGGCCCGGGGCATCCGCCGGGTGCAGATCCTGAGCCTCCGCCGCAGCCTCAGCTACGTGATCCAGGGCATGGCCAACATCGAGAGCCTCAACCTCAGCGGCTGCTACAACCTCACCGACAACGGGCTGGGCCACGCGTTTGTGCAGGAGATCGGCTCCCTGCGCGCTCTCAACCTGAGCCTCTGCAAGCAGATCACTGACAGCAGCCTGGGCCGCATAGCCCAGTACCTCAAGGGCCTGGAGGTGCTGGAGCTGGGAGGTTGCAGCAACATCACCAACACTGGCCTTCTGCTCATCGCCTGGGGTCTGCAGCGCCTCAAGAGCCTTAACCTCCGCAGCTGCCGCCACCTTTCGGATGTGGGCATCGGGCACCTGGCCGGCATGACGCGCAGCGCGGCGGAGGGCTGCCTGGGCCTGGAGCAGCTCACGCTACAGGACTGCCAGAAGCTCACAGATCTTTCTCTAAAGCACATCTCCCGAGGGCTGACGGGCCTGAGGCTCCTCAACCTCAGCTTCTGTGGGGGAATCTCGGACGCTGGCCTCCTGCACCTGTCGCACATGGGCAGCCTGCGCAGCCTCAACCTGCGCTCCTGTGACAACATCAGTGACACGGGCATCATGCATCTGGCCATGGGCAGCCTGCGCCTCTCGGGGCTGGATGTTTCGTTCTGTGACAAGGTGGGAGACCAGAGTCTGGCTTACATAGCCCAGGGGCTGGATGGCCTCAAGTCTCTCTCCCTCTGCTCCTGCCACATCAGTGATGATGGCATCAACCGCATGGTGCGGCAGATGCACGGGCTGCGCACGCTCAACATTGGACAGTGTGTGCGCATCACGGACAAGGGCCTGGAGCTGATCGCTGAGCACCTGAGCCAACTCACCGGCATAGACCTGTACGGCTGCACCCGAATCACCAAGCGCGGCCTGGAGCGCATCACGCAGCTGCCGTGCCTCAAGGTACTCAACCTGGGACTCTGGCAGATGACGGACAGTGAGAAGGAGGCACGAGGGGATTTTTCTCCATTATTCACTGTGAGAACTCGGGGAAGCTCCAGAAGGTGA Translation Sequence: METHISCLFP ELLAMIFGYL DVRDKGRAAQ VCTAWRDAAY HKSVWRGVEA KLHLRRANPS LFPSLQARGI RRVQILSLRR SLSYVIQGMA NIESLNLSGC YNLTDNGLGH AFVQEIGSLR ALNLSLCKQI TDSSLGRIAQ YLKGLEVLEL GGCSNITNTG LLLIAWGLQR LKSLNLRSCR HLSDVGIGHL AGMTRSAAEG CLGLEQLTLQ DCQKLTDLSL KHISRGLTGL RLLNLSFCGG ISDAGLLHLS HMGSLRSLNL RSCDNISDTG IMHLAMGSLR LSGLDVSFCD KVGDQSLAYI AQGLDGLKSL SLCSCHISDD GINRMVRQMH GLRTLNIGQC VRITDKGLEL IAEHLSQLTG IDLYGCTRIT KRGLERITQL PCLKVLNLGL WQMTDSEKEA RGDFSPLFTV RTRGSSRR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
