Fibrinogen silencer-binding protein, also known as FSBP, is interacts with X11alpha. X11alpha/FSBP complex signals to the nucleus to repress glycogen synthase kinase-3beta promoter activity. X11alpha is a neuronal adaptor protein that interacts with the amyloid precursor protein (APP) through a centrally located phosphotyrosine binding domain to inhibit the production of Abeta peptide that is deposited in Alzheimers disease brains. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 900bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGTAGGAAAGGCTAGATCTTCCAATTTTACCTTATCCGAAAAGCTTGATTTGCTAAAGCTTGTGAAGCCATATGTGAAAATTCTCGAAGAACACACTAATAAACATTCAGTAATAGTAGAAAAGAATAGATGTTGGGATATCATAGCAGTTAACTATAATGCAATTGGAGTAGACCGCCCTCCTCGAACAGCACAGGGCCTACGCACCCTTTATAAAAGGCTCAAAGAATATGCCAAACAGGAGCTATTGCAGCAAAAAGAGACCCAATCAGATTTTAAAAGCAATATTTCTGAGCCAACCAAGAAAGTTATGGAGATGATTCCCCAGATTTCCAGTTTTTGCCTGGTAAGAGACAGGAACCACATACAAAGTGCAAACTTGGATGAGGAGGCACAGGCTGGTACCAGTTCACTACAGGTAATGTTGGATCACCATCCTGTTGCTATTACAGTGGAGGTGAAGCAAGAAGAAGACATTAAACCACCTCCTCCACTGGTTTTAAATTCTCAACAGAGTGATACTTTAGAGCAAAGAGAAGAACATGAATTAGTACATGTTATGGAAAGATCCTTGTCGCCTTCACTTTCCTCTGTTGATATGAGAATGACATCGTCTCCATCTTCTATTCCAAGGAGAGATGATTTTTTTCGGCATGAGAGTGGTGAACACTTTAGGTCACTATTAGGGTATGATCCTCAGATCCTGCAAATGTTGAAAGAGGAGCATCAGATAATTTTAGAAAATCAAAAAAATTTTGGATTGTATGTTCAGGAGAAGAGGGATGGATTGAAAAGAAGGCAGCAGCTAGAGGAAGAGCTACTAAGAGCAAAAATTGAAGTGGAGAAGCTGAAAGCAATTCGCTTACGGCATGATCTACCTGAATATAACAGTCTCTAA Translation Sequence: MVGKARSSNF TLSEKLDLLK LVKPYVKILE EHTNKHSVIV EKNRCWDIIA VNYNAIGVDRPPRTAQGLRT LYKRLKEYAK QELLQQKETQ SDFKSNISEP TKKVMEMIPQ ISSFCLVRDRNHIQSANLDE EAQAGTSSLQ VMLDHHPVAI TVEVKQEEDI KPPPPLVLNS QQSDTLEQREEHELVHVMER SLSPSLSSVD MRMTSSPSSI PRRDDFFRHE SGEHFRSLLG YDPQILQMLKEEHQIILENQ KNFGLYVQEK RDGLKRRQQL EEELLRAKIE VEKLKAIRLR HDLPEYNSL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
