GuK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GuK1 is thought to participate in regulating the supply of guanine nucleotides to signal transduction pathways. Overexpression of GuK1 is associated with pituitary adenocarcinomas, suggesting that GuK1 is involved in tumorigenesis. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 594bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTCGGGCC CCAGGCCTGT GGTGCTGAGC GGGCCTTCGG GAGCTGGGAA GAGCACCCTG CTGAAGAGGC TGCTCCAGGA GCACAGCGGC ATCTTTGGCT TCAGCGTGTC CCATACCACG AGGAACCCGA GGCCCGGCGA GGAGAACGGC AAAGATTACT ACTTTGTAAC CAGGGAGGTG ATGCAGCGTG ACATAGCAGC CGGCGACTTC ATCGAGCATG CCGAGTTCTC GGGGAACCTG TATGGCACGA GCAAGGTGGC GGTGCAGGCC GTGCAGGCCA TGAACCGCAT CTGTGTGCTG GACGTGGACC TGCAGGGTGT GCGGAACATC AAGGCCACCG ATCTGCGGCC CATCTACATC TCTGTGCAGC CGCCTTCACT GCACGTGCTG GAGCAGCGGC TGCGGCAGCG CAACACTGAA ACCGAGGAGA GCCTGGTGAA GCGGCTGGCT GCTGCCCAGG CCGACATGGA GAGCAGCAAG GAGCCCGGCC TGTTTGATGT GGTCATCATT AACGACAGCC TGGACCAGGC CTACGCAGAG CTGAAGGAGG CGCTCTCTGA GGAAATCAAG AAAGCTCAAA GGACCGGCGC CTGA Translation Sequence: MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
