Interleukin 9, also known as IL-9, is a cytokine (cell signalling molecule) belonging to the group of interleukins. This protein produced by T-cells and specifically by CD4+ helper cells that acts as a regulator of a variety of hematopoietic cells and stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 435bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCTTCTGG CCATGGTCCT TACCTCTGCC CTGCTCCTGT GCTCCGTGGC AGGCCAGGGG TGTCCAACCT TGGCGGGGAT CCTGGACATC AACTTCCTCA TCAACAAGAT GCAGGAAGAT CCAGCTTCCA AGTGCCACTG CAGTGCTAAT GTGACCAGTT GTCTCTGTTT GGGCATTCCC TCTGACAACT GCACCAGACC ATGCTTCAGT GAGAGACTGT CTCAGATGAC CAATACCACC ATGCAAACAA GATACCCACT GATTTTCAGT CGGGTGAAAA AATCAGTTGA AGTACTAAAG AACAACAAGT GTCCATATTT TTCCTGTGAA CAGCCATGCA ACCAAACCAC GGCAGGCAAC GCGCTGACAT TTCTGAAGAG TCTTCTGGAA ATTTTCCAGA AAGAAAAGAT GAGAGGGATG AGAGGCAAGA TATGA Translation Sequence: MLLAMVLTSA LLLCSVAGQG CPTLAGILDI NFLINKMQED PASKCHCSAN VTSCLCLGIP SDNCTRPCFS ERLSQMTNTT MQTRYPLIFS RVKKSVEVLK NNKCPYFSCE QPCNQTTAGN ALTFLKSLLE IFQKEKMRGM RGKI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
