IMPACT belongs to the IMPACT family and contains 1 RWD domain. It is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 963bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCTGAGGGGGACGCAGGGAGCGACCAGAGGCAGAATGAGGAAATTGAAGCAATGGCAGCCATTTATGGCGAGGAGTGGTGTGTCATTGATGACTGTGCCAAAATATTTTGTATTAGAATTAGCGACGATATAGATGACCCCAAATGGACACTTTGCTTGCAGGTGATGCTGCCGAATGAATACCCAGGTACAGCTCCACCTATCTACCAGTTGAATGCTCCTTGGCTTAAAGGGCAAGAACGTGCGGATTTATCAAATAGCCTTGAGGAAATATATATTCAGAATATCGGTGAAAGTATTCTTTACCTGTGGGTGGAGAAAATAAGAGATGTTCTTATACAAAAATCTCAGATGACAGAACCAGGCCCAGATGTAAAGAAGAAAACTGAAGAGGAAGATGTTGAATGTGAAGATGATCTCATTTTAGCATGTCAGCCGGAAAGTTCGGTTAAAGCATTGGATTTTGATATCAGTGAAACTCGGACAGAAGTAGAAGTAGAAGAATTACCTCCGATTGATCATGGCATTCCTATTACAGACCGAAGAAGTACTTTTCAGGCACACTTGGCTCCAGTGGTTTGTCCCAAACAGGTGAAAATGGTTCTTTCCAAATTGTATGAGAATAAGAAAATAGCTAGTGCCACCCACAACATCTATGCCTACAGAATATATTGTGAGGATAAACAGACCTTCTTACAGGATTGTGAGGATGATGGGGAAACAGCAGCTGGTGGGCGTCTTCTTCATCTCATGGAGATTTTGAATGTGAAGAATGTCATGGTGGTAGTATCACGCTGGTATGGAGGGATTCTGCTAGGACCAGATCGCTTTAAACATATCAACAACTGTGCCAGAAACATACTAGTGGAAAAGAACTACACAAATTCACCTGAGGAGTCATCTAAGGCTTTGGGAAAGAACAAAAAAGTAAGAAAAGACAAGAAGAGGAATGAACATTAA Translation Sequence: MAEGDAGSDQ RQNEEIEAMA AIYGEEWCVI DDCAKIFCIR ISDDIDDPKW TLCLQVMLPN EYPGTAPPIY QLNAPWLKGQ ERADLSNSLE EIYIQNIGES ILYLWVEKIR DVLIQKSQMT EPGPDVKKKT EEEDVECEDD LILACQPESS VKALDFDISE TRTEVEVEEL PPIDHGIPIT DRRSTFQAHL APVVCPKQVK MVLSKLYENK KIASATHNIY AYRIYCEDKQ TFLQDCEDDG ETAAGGRLLH LMEILNVKNV MVVVSRWYGG ILLGPDRFKH INNCARNILV EKNYTNSPEE SSKALGKNKK VRKDKKRNEH Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
