JAZF1 is a nuclear protein with three C2H2-type zinc fingers, and functions as a transcriptional repressor. Chromosomal aberrations involving JAZF1 are associated with endometrial stromal tumors. Alternatively spliced variants which encode different protein isoforms have been described, however, not all variants have been fully characterized. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 732bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGACAGGCATCGCCGCCGCCTCCTTCTTCTCCAATACCTGCCGATTCGGGGGCTGCGGACTCCACTTCCCCACCCTGGCCGACCTCATCGAGCACATCGAGGACAACCACATCGATACAGATCCACGGGTTTTAGAAAAACAAGAATTACAGCAGCCAACCTATGTTGCCCTGAGTTACATAAATAGATTCATGACAGATGCTGCCCGCCGAGAGCAGGAGTCCCTAAAGAAGAAGATTCAGCCGAAGCTCTCGCTGACTCTGTCCAGCTCAGTGTCTCGAGGGAATGTGTCCACTCCCCCACGCCACAGCAGTGGAAGCCTTACTCCCCCCGTGACCCCACCCATCACCCCCTCCTCTTCATTCCGCAGCAGCACTCCGACAGGCAGCGAGTATGACGAGGAGGAGGTGGACTATGAGGAGTCGGACAGCGATGAGTCCTGGACCACAGAGAGTGCCATCAGCTCCGAAGCCATCCTCAGCTCCATGTGCATGAATGGAGGGGAAGAGAAGCCTTTTGCCTGCCCAGTTCCTGGATGTAAAAAGAGATACAAGAATGTGAATGGCATAAAGTATCACGCTAAGAATGGTCACAGAACACAGATTCGTGTCCGCAAACCATTCAAGTGTCGCTGTGGGAAGAGTTACAAGACAGCTCAGGGCCTGCGGCACCACACAATCAATTTCCATCCCCCGGTGTCGGCTGAGATTATCAGGAAGATGCAGCAATAA Translation Sequence: MTGIAAASFF SNTCRFGGCG LHFPTLADLI EHIEDNHIDT DPRVLEKQEL QQPTYVALSYINRFMTDAAR REQESLKKKI QPKLSLTLSS SVSRGNVSTP PRHSSGSLTP PVTPPITPSSSFRSSTPTGS EYDEEEVDYE ESDSDESWTT ESAISSEAIL SSMCMNGGEE KPFACPVPGCKKRYKNVNGI KYHAKNGHRT QIRVRKPFKC RCGKSYKTAQ GLRHHTINFH PPVSAEIIRKMQQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
