LSM12 belongs to the snRNP Sm proteins family. Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 588bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCGGCTCCTCCGGGCGAGTACTTCAGCGTTGGGAGCCAGGTGTCGTGCCGGACGTGCCAGGAGCAGCGGCTGCAGGGCGAGGTGGTAGCCTTTGACTACCAATCCAAAATGCTGGCTTTAAAATGTCCCTCTTCCAGTGGAAAGCCCAACCATGCAGACATCTTGCTCATAAACTTACAGTATGTTTCAGAAGTGGAAATAATTAATGACCGAACAGAAACCCCTCCTCCCCTAGCTTCACTCAATGTTAGTAAGCTTGCCAGCAAAGCACGGACAGAGAAGGAGGAGAAGCTGAGCCAGGCCTATGCAATCAGTGCTGGTGTCTCTCTAGAGGGCCAGCAGCTCTTCCAGACCATTCACAAGACCATTAAAGACTGTAAATGGCAAGAAAAAAACATCGTAGTCATGGAAGAAGTTGTTATTACACCCCCATATCAAGTGGAAAACTGTAAAGGCAAAGAGGGGAGTGCACTGAGCCATGTACGCAAAATAGTTGAAAAACATTTTAGAGACGTGGAAAGCCAAAAGATACTGCAACGTTCACAAGCCCAGCAACCACAGAAGGAGGCTGCCCTGTCATCCTGA Translation Sequence: MAAPPGEYFS VGSQVSCRTC QEQRLQGEVV AFDYQSKMLA LKCPSSSGKP NHADILLINL QYVSEVEIIN DRTETPPPLA SLNVSKLASK ARTEKEEKLS QAYAISAGVS LEGQQLFQTI HKTIKDCKWQ EKNIVVMEEV VITPPYQVEN CKGKEGSALS HVRKIVEKHF RDVESQKILQ RSQAQQPQKE AALSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
