The protein encoded by MOAP1 was identified by its interaction with apoptosis regulator BAX protein. This protein contains a Bcl-2 homology 3 (BH3) -like motif, which is required for the association with BAX. When overexpressed, this gene has been shown to mediate caspase-dependent apoptosis. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1056bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGACTTTGAGGCTTTTAGAAGACTGGTGCAGGGGGATGGACATGAACCCTCGGAAAGCGCTATTGATTGCCGGCATCTCCCAGAGCTGCAGTGTGGCAGAAATCGAGGAGGCTCTGCAGGCTGGTTTAGCTCCCTTGGGGGAGTACAGACTGCTTGGAAGGATGTTCAGGAGGGATGAGAACAGGAAAGTAGCCTTAGTAGGGCTTACTGCGGAGACTAGTCACGCCCTGGTCCCTAAGGAGATACCGGGAAAAGGGGGTATCTGGAGAGTGATCTTTAAGCCCCCTGACCCAGATAATACATTTTTAAGCAGATTAAATGAATTTTTAGCGGGAGAGGGCATGACAGTGGGTGAGTTGAGCAGAGCTCTTGGACATGAAAATGGCTCCTTAGACCCAGAGCAGGGCATGATCCCGGAAATGTGGGCCCCTATGTTGGCACAGGCATTAGAGGCTCTTCAGCCTGCCCTGCAATGCTTGAAGTATAAAAAGCTGAGAGTGTTCTCGGGCAGGGAGTCTCCAGAACCAGGAGAAGAAGAATTTGGACGCTGGATGTTTCATACTACTCAGATGATAAAGGCGTGGCAGGTGCCAGATGTAGAGAAGAGAAGGCGATTGCTAGAGAGCCTTCGAGGCCCAGCACTTGATGTTATTCGTGTCCTCAAGATAAACAATCCTTTAATTACTGTCGATGAATGTCTGCAGGCTCTTGAGGAGGTATTTGGGGTTACAGATAATCCTAGGGAGTTGCAGGTCAAATATCTAACCACTTACCAGAAGGATGAGGAAAAGTTGTCGGCTTATGTACTAAGGCTGGAGCCTTTGTTACAGAAGCTGGTACAGAGAGGAGCAATTGAGAGAGATGCTGTGAATCAGGCCCGCCTAGACCAAGTCATTGCTGGGGCAGTCCACAAAACAATTCGCAGAGAGCTTAATCTGCCAGAGGATGGCCCAGCCCCTGGTTTCTTGCAGTTATTGGTACTAATAAAGGATTATGAGGCAGCTGAGGAGGAGGAGGCCCTTCTCCAGGCAATATTGGAAGGTAATTTCACCTGA Translation Sequence: MTLRLLEDWC RGMDMNPRKA LLIAGISQSC SVAEIEEALQ AGLAPLGEYR LLGRMFRRDE NRKVALVGLT AETSHALVPK EIPGKGGIWR VIFKPPDPDN TFLSRLNEFL AGEGMTVGEL SRALGHENGS LDPEQGMIPE MWAPMLAQAL EALQPALQCL KYKKLRVFSG RESPEPGEEE FGRWMFHTTQ MIKAWQVPDV EKRRRLLESL RGPALDVIRV LKINNPLITV DECLQALEEV FGVTDNPREL QVKYLTTYQK DEEKLSAYVL RLEPLLQKLV QRGAIERDAV NQARLDQVIA GAVHKTIRRE LNLPEDGPAP GFLQLLVLIK DYEAAEEEEA LLQAILEGNF T Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
