MRTO4 encodes a protein sharing a low level of sequence similarity with ribosomal protein P0. While the precise function of the encoded protein is currently unknown, it appears to be involved in mRNA turnover and ribosome assembly. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 720bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCCCAAATCCAAGCGCGACAAGAAAGTCTCCTTAACCAAAACTGCCAAGAAAGGCTTGGAATTGAAACAAAACCTGATAGAAGAGCTTCGGAAATGTGTGGACACCTACAAGTACCTTTTCATCTTCTCTGTGGCCAACATGAGGAACAGCAAGCTGAAGGACATCCGGAACGCCTGGAAGCACAGCCGGATGTTCTTTGGCAAAAACAAGGTGATGATGGTGGCCTTGGGTCGGAGCCCATCTGATGAATACAAAGACAACCTGCACCAGGTCAGCAAAAGGTTGAGGGGTGAGGTGGGTCTCCTGTTCACCAACCGCACAAAGGAGGAGGTGAATGAGTGGTTCACGAAATACACAGAAATGGACTACGCCCGAGCTGGTAACAAAGCAGCTTTCACTGTGAGCCTGGATCCAGGGCCCCTGGAGCAGTTCCCCCACTCCATGGAGCCACAGCTCAGGCAGCTGGGCCTGCCCACCGCCCTCAAGAGAGGTGTGGTGACTCTGCTGTCTGACTACGAGGTGTGCAAGGAGGGCGATGTGCTGACCCCAGAGCAGGCTCGCGTCCTGAAGCTTTTTGGGTATGAGATGGCTGAATTCAAGGTGACCATCAAATACATGTGGGATTCACAGTCGGGAAGGTTCCAGCAGATGGGAGACGACTTGCCAGAGAGCGCATCTGAGTCCACAGAAGAGTCAGACTCAGAAGATGATGACTGA Translation Sequence: MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNLH QVSKRLRGEV GLLFTNRTKE EVNEWFTKYT EMDYARAGNK AAFTVSLDPG PLEQFPHSME PQLRQLGLPT ALKRGVVTLL SDYEVCKEGD VLTPEQARVL KLFGYEMAEF KVTIKYMWDS QSGRFQQMGD DLPESASEST EESDSEDDD Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
