NAA30 (N (Alpha) -Acetyltransferase 30, NatC Catalytic Subunit) is a catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. It catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. NAA30 is necessary for the lysosomal localization and function of ARL8B sugeesting that ARL8B is a NatC substrate. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1089bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCGGAGGTACCGCCTGGGCCTAGCAGCCTCCTCCCACCACCAGCACCTCCGGCCCCGGCGGCGGTCGAGCCCCGCTGTCCCTTCCCGGCGGGGGCCGCCCTCGCCTGCTGCAGCGAGGACGAGGAGGACGACGAAGAGCACGAAGGCGGCGGCAGCAGGAGCCCGGCGGGCGGAGAGTCGGCGACGGTGGCGGCCAAGGGGCATCCGTGCCTCCGCTGCCCTCAGCCGCCGCAGGAGCAGCAGCAGCTCAACGGATTGATTAGCCCCGAACTGCGGCACCTCCGGGCGGCCGCCTCCCTCAAGAGCAAGGTCCTGAGCGTAGCAGAGGTGGCCGCGACCACAGCCACCCCTGACGGAGGCCCCAGAGCGACTGCAACAAAAGGAGCCGGGGTACACTCGGGCGAGAGGCCCCCTCACTCCCTCTCTAGTAATGCAAGAACTGCGGTCCCCAGCCCGGTGGAGGCAGCGGCGGCGAGCGATCCCGCGGCGGCCCGCAATGGACTGGCCGAGGGCACCGAGCAGGAGGAGGAGGAGGAAGACGAGCAGGTGCGGCTGCTGTCTTCGTCCCTGACCGCCGACTGCAGCTTAAGAAGCCCTTCGGGCAGGGAGGTTGAGCCTGGGGAGGATCGGACGATACGATATGTCCGATATGAATCCGAGCTACAAATGCCCGATATCATGAGACTGATCACCAAAGATCTGTCCGAACCCTACTCCATTTATACCTATAGATATTTTATCCACAACTGGCCACAGCTGTGCTTCTTGGCCATGGTAGGGGAGGAGTGTGTAGGTGCCATCGTTTGCAAGTTGGATATGCACAAAAAGATGTTCCGCAGAGGTTATATAGCCATGTTAGCCGTGGATTCCAAATACAGGAGAAATGGCATTGGTACTAACTTGGTTAAGAAAGCTATATATGCCATGGTTGAGGGAGACTGTGATGAGGTTGTTTTGGAAACCGAAATAACAAATAAGTCCGCTTTGAAACTTTATGAAAATCTTGGTTTTGTTCGAGATAAGAGGCTGTTCAGATACTATTTAAATGGAGTTGATGCACTGCGACTTAAACTGTGGCTGCGTTGA Translation Sequence: MAEVPPGPSS LLPPPAPPAP AAVEPRCPFP AGAALACCSE DEEDDEEHEG GGSRSPAGGESATVAAKGHP CLRCPQPPQE QQQLNGLISP ELRHLRAAAS LKSKVLSVAE VAATTATPDGGPRATATKGA GVHSGERPPH SLSSNARTAV PSPVEAAAAS DPAAARNGLA EGTEQEEEEEDEQVRLLSSS LTADCSLRSP SGREVEPGED RTIRYVRYES ELQMPDIMRL ITKDLSEPYSIYTYRYFIHN WPQLCFLAMV GEECVGAIVC KLDMHKKMFR RGYIAMLAVD SKYRRNGIGTNLVKKAIYAM VEGDCDEVVL ETEITNKSAL KLYENLGFVR DKRLFRYYLN GVDALRLKLWLR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
