NACC1 is a member of the BTB/POZ protein family. BTB/POZ proteins are involved in several cellular processes including proliferation, apoptosis and transcription regulation. It is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. A pseudogene of this gene is located on the short arm of chromosome 9. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1029bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCGGAAGGAAACCCCACCCCCCCTAGTGCCCCCGGCGGCCCGGGAGTGGAATCTTCCCCCAAATGCGCCCGCCTGCATGGAACGGCAGTTGGAGGCTGCGCGGTACCGGTCCGATGGGGCGCTTCTCCTCGGGGCCTCCAGCCTGAGTGGGCGCTGCTGGGCCGGCTCCCTCTGGCTTTTTAAGGACCCCTGTGCCGCCCCCAACGAAGGCTTCTGCTCCGCCGGAGTCCAAACGGAGGCTGGAGTGGCTGACCTCACTTGGGTTGGGGAGAGAGGTATTCTAGTGGCCTCCGATTCAGGTGCTGTTGAATTGTGGGAACTAGATGAGAATGAGACACTTATTGTCAGCAAGTTCTGCAAGTATGAGCATGATGACATTGTGTCTACAGTCAGTGTCTTGAGCTCTGGCACACAAGCTGTCAGTGGTAGCAAAGACATCTGCATCAAGGTTTGGGACCTTGCTCAGCAGGTGGTACTGAGTTCATACCGAGCTCATGCTGCTCAGGTCACTTGTGTTGCTGCCTCTCCTCACAAGGACTCTGTGTTTCTTTCATGCAGCGAGGACAATAGAATTTTACTCTGGGATACCCGCTGTCCCAAGCCAGCATCACAGATTGGCTGCAGTGCGCCTGGCTACCTTCCTACCTCGCTGGCTTGGCATCCTCAGCAAAGTGAAGTCTTTGTCTTTGGTGATGAGAATGGGACAGTCTCCCTTGTGGACACCAAGAGTACAAGCTGTGTCCTGAGCTCAGCTGTACACTCCCAGTGTGTCACTGGGCTGGTGTTCTCCCCACACAGTGTTCCCTTCCTGGCCTCTCTCAGTGAAGACTGCTCACTTGCTGTGCTGGACTCAAGCCTTTCTGAGTTGTTTAGAAGCCAAGCCCACAGAGACTTTGTGAGAGATGCGACTTGGTCCCCGCTCAATCACTCCCTGCTTACCACAGTGGGCTGGGACCATCAGGTCGTCCACCACGTTGTGCCCACAGAACCTCTCCCAGCCCCTGGACCTGCAAGTGTTACTGAGTAG Translation Sequence: MRKETPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG ALLLGASSLS GRCWAGSLWL FKDPCAAPNE GFCSAGVQTE AGVADLTWVG ERGILVASDS GAVELWELDE NETLIVSKFC KYEHDDIVST VSVLSSGTQA VSGSKDICIK VWDLAQQVVL SSYRAHAAQV TCVAASPHKD SVFLSCSEDN RILLWDTRCP KPASQIGCSA PGYLPTSLAW HPQQSEVFVF GDENGTVSLV DTKSTSCVLS SAVHSQCVTG LVFSPHSVPF LASLSEDCSL AVLDSSLSEL FRSQAHRDFV RDATWSPLNH SLLTTVGWDH QVVHHVVPTE PLPAPGPASV TE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
