PARD6B is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cytoplasmic protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1119bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAACCGCAGCCACCGGCACGGGGCGGGCAGCGGCTGCCTGGGCACTATGGAGGTGAAGAGCAAGTTTGGAGCTGAATTTCGTCGGTTTTCGCTGGAAAGATCAAAACCTGGAAAATTTGAGGAGTTTTATGGATTACTACAACATGTTCATAAGATCCCCAATGTTGACGTTTTGGTAGGCTATGCAGACATCCATGGAGACTTACTACCTATAAATAATGATGATAATTATCACAAAGCTGTTTCAACGGCCAATCCACTGCTTAGGATATTTATACAAAAGAAGGAAGAAGCAGACTACAGTGCCTTTGGTACAGACACGCTAATAAAGAAGAAGAATGTTTTAACCAACGTATTGCGTCCTGACAACCATAGAAAAAAGCCACATATAGTCATTAGTATGCCCCAAGACTTTAGACCTGTGTCTTCTATTATAGACGTGGATATTCTCCCAGAAACGCATCGTAGGGTACGTCTTTACAAATACGGCACGGAGAAACCCCTAGGATTCTACATCCGGGATGGCTCCAGTGTCAGGGTAACACCACATGGCTTAGAAAAGGTTCCAGGGATCTTTATATCCAGGCTTGTCCCAGGAGGTCTGGCTCAAAGTACAGGACTATTAGCTGTTAATGATGAAGTTTTAGAAGTTAATGGCATAGAAGTTTCAGGGAAGAGCCTTGATCAAGTAACAGACATGATGATTGCAAATAGCCGTAACCTCATCATAACAGTGAGACCGGCAAACCAGAGGAATAATGTTGTGAGGAACAGTCGGACTTCTGGCAGTTCCGGTCAGTCTACTGATAACAGCCTTCTTGGCTACCCACAGCAGATTGAACCAAGCTTTGAGCCAGAGGATGAAGACAGCGAAGAAGATGACATTATCATTGAAGACAATGGAGTGCCACAGCAGATTCCAAAAGCTGTTCCTAATACTGAGAGCCTGGAGTCATTAACACAGATAGAGCTAAGCTTTGAGTCTGGACAGAATGGCTTTATTCCCTCTAATGAAGTGAGCTTAGCAGCCATAGCAAGCAGCTCAAACACGGAATTTGAAACACATGCTCCAGATCAAAAACTCTTAGAAGAAGATGGAACAATCATAACATTATGA Translation Sequence: MNRSHRHGAG SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV HKIPNVDVLVGYADIHGDLL PINNDDNYHK AVSTANPLLR IFIQKKEEAD YSAFGTDTLI KKKNVLTNVLRPDNHRKKPH IVISMPQDFR PVSSIIDVDI LPETHRRVRL YKYGTEKPLG FYIRDGSSVRVTPHGLEKVP GIFISRLVPG GLAQSTGLLA VNDEVLEVNG IEVSGKSLDQ VTDMMIANSRNLIITVRPAN QRNNVVRNSR TSGSSGQSTD NSLLGYPQQI EPSFEPEDED SEEDDIIIEDNGVPQQIPKA VPNTESLESL TQIELSFESG QNGFIPSNEV SLAAIASSSN TEFETHAPDQKLLEEDGTII TL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
