PCOTH (Prostate collagen triple helix protein) is a 107aa cytoplasmic protein that is specifically expressed in prostate and testis. PCOTH may play a significant role in the proliferation and viability of prostate cancers by way of phosphorylating the oncoprotein I2PP2A. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 324bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTGGATCTTATCAAATTTAATGGGCACATCTGAAGAAGGAAACTTGCTCAGCACCGTGAGCCCCACAGTGAAAGCACTTTTTGGCAAGACTAGAGTCTCACCGATTTTCCCTTTCTCTCCTCGATCTCCTTTCCAGCCTCTTATTCCCCGGACTCCTGGCTCACCCTGGGGCCCCGTGGGTCCAGCTTCTCCCTTGGGACCAGGCTTTCCAATAGGGCCCATGGGGCCCGGTAAACCAGTTGGGCCCAAAGGCCCAATGTTGCCCCTTGGCCCCTCAGGACCAGTGGGACCCACGTCACCCTTATTCCCCTTCTGCCCCTGA Translation Sequence: MWILSNLMGT SEEGNLLSTV SPTVKALFGK TRVSPIFPFS PRSPFQPLIP RTPGSPWGPVGPASPLGPGF PIGPMGPGKP VGPKGPMLPL GPSGPVGPTS PLFPFCP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
