PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases by similarity. This protein functions as an estrogen receptor (ER) -selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. PHB2 competes with NCOA1 for modulation of ER transcriptional activity. It is probably involved in regulating mitochondrial respiration activity and in aging. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 900bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCCCAGA ACTTGAAGGA CTTGGCGGGA CGGCTGCCCG CCGGGCCCCG GGGCATGGGC ACGGCCCTGA AGCTGTTGCT GGGGGCCGGC GCCGTGGCCT ACGGTGTGCG CGAATCTGTG TTCACCGTGG AAGGCGGGCA CAGAGCCATC TTCTTCAATC GGATCGGTGG AGTGCAGCAG GACACTATCC TGGCCGAGGG CCTTCACTTC AGGATCCCTT GGTTCCAGTA CCCCATTATC TATGACATTC GGGCCAGACC TCGAAAAATC TCCTCCCCTA CAGGCTCCAA AGACCTACAG ATGGTGAATA TCTCCCTGCG AGTGTTGTCT CGACCCAATG CTCAGGAGCT TCCTAGCATG TACCAGCGCC TAGGGCTGGA CTACGAGGAA CGAGTGTTGC CGTCCATTGT CAACGAGGTG CTCAAGAGTG TGGTGGCCAA GTTCAATGCC TCACAGCTGA TCACCCAGCG GGCCCAGGTA TCCCTGTTGA TCCGCCGGGA GCTGACAGAG AGGGCCAAGG ACTTCAGCCT CATCCTGGAT GATGTGGCCA TCACAGAGCT GAGCTTTAGC CGAGAGTACA CAGCTGCTGT AGAAGCCAAA CAAGTGGCCC AGCAGGAGGC CCAGCGGGCC CAATTCTTGG TAGAAAAAGC AAAGCAGGAA CAGCGGCAGA AAATTGTGCA GGCCGAGGGT GAGGCCGAGG CTGCCAAGAT GCTTGGAGAA GCACTGAGCA AGAACCCTGG CTACATCAAA CTTCGCAAGA TTCGAGCAGC CCAGAATATC TCCAAGACGA TCGCCACATC ACAGAATCGT ATCTATCTCA CAGCTGACAA CCTTGTGCTG AACCTACAGG ATGAAAGTTT CACCAGGGGA AGTGACAGCC TCATCAAGGG TAAGAAATGA Translation Sequence: MAQNLKDLAG RLPAGPRGMG TALKLLLGAG AVAYGVRESV FTVEGGHRAI FFNRIGGVQQ DTILAEGLHF RIPWFQYPII YDIRARPRKI SSPTGSKDLQ MVNISLRVLS RPNAQELPSM YQRLGLDYEE RVLPSIVNEV LKSVVAKFNA SQLITQRAQV SLLIRRELTE RAKDFSLILD DVAITELSFS REYTAAVEAK QVAQQEAQRA QFLVEKAKQE QRQKIVQAEG EAEAAKMLGE ALSKNPGYIK LRKIRAAQNI SKTIATSQNR IYLTADNLVL NLQDESFTRG SDSLIKGKK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
