Prostatic Acid Phosphatase, Recombinant, Mouse, aa32-381, His-Tag

Produktübersicht

• Artikelname: Prostatic Acid Phosphatase, Recombinant, Mouse, aa32-381, His-Tag
• Artikelnummer: USB-552196
• Hersteller Artikelnummer: 552196
• Alternativnummer: USB-552196-50,USB-552196-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

ACPP, also known as Prostatic Acid Phosphatase, is a member of histidine acid phosphatase family. Prostatic Acid Phosphatase/ACPP catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins. The activity optimum of Prostatic Acid Phosphatase/ACPP is in the pH range of 4 - 6, and the activity is inhibited by L(+)-tartrate. It has also lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Recombinant protein corresponding to aa32-381 from mouse Prostatic Acid Phosphatase, fused to His-Tag at C-terminal, expressed in baculovirus. Amino Acid Sequence: KELKFVTLVF RHGDRGPIET FPTDPITESS WPQGFGQLTQ WGMEQHYELG SYIRKRYGRF LNDTYKHDQI YIRSTDVDRT LMSAMTNLAA LFPPEGISIW NPRLLWQPIP VHTVSLSEDR LLYLPFRDCP RFEELKSETL ESEEFLKRLH PYKSFLDTLS SLSGFDDQDL FGIWSKVYDP LFCESVHNFT LPSWATEDAM IKLKELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILKNMK LATQPQKYKK LVMYSAHDTT VSGLQMALDV YNGVLPPYAS CHMMELYHDK GGHFVEMYYR NETQNEPYPL TLPGCTHSCP LEKFAELLDP VISQDWATEC MATSSHQGRN Biological Activity: Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of pnitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 41.3
• NCBI: 062781
• UniProt: Q8CE08
• Reinheit: 95% (SDS-PAGE). Endotoxin: <1EU/1ug (LAL)
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.