S100 calcium binding protein Z, also known as S100Z, is member of the S100 protein family. S100 proteins are small dimeric members of the EF-hand superfamily of Ca (2+) binding proteins thought to participate in mediating intracellular Ca (2+) signals by binding to and thereby regulating target proteins in a Ca (2+) -dependent manner. There are differences in the expression level of S100Z mRNA in various tissues. The highest levels were found in spleen and leukocytes. S100Z gene expression appears to be deregulated in some tumor tissues, compared to expression in their normal counterparts. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 300bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCCCACCC AGCTCGAGAT GGCCATGGAC ACCATGATTA GAATCTTCCA CCGCTATTCT GGCAAGGAAA GGAAGAGATT CAAGCTCAGC AAGGGGGAAC TGAAACTGCT CCTGCAGCGA GAGCTCACGG AATTCCTCTC GTGCCAAAAG GAAACCCAGT TGGTTGATAA GATAGTGCAG GACCTGGATG CCAATAAGGA CAACGAAGTG GATTTTAATG AATTCGTGGT CATGGTGGCA GCTCTGACAG TTGCTTGTAA TGATTACTTT GTAGAACAAT TGAAGAAGAA AGGAAAATAA Translation Sequence: MPTQLEMAMD TMIRIFHRYS GKERKRFKLS KGELKLLLQR ELTEFLSCQK ETQLVDKIVQ DLDANKDNEV DFNEFVVMVA ALTVACNDYF VEQLKKKGK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
