SHP-2, also known as, tyrosine-protein phospatase non-receptor type 11(PTPN11), is a member of the protein tyrosine phosphatase (PTP) family containing two Src homology 2 domains. This protein dephosphorylates tyrosine residues in proteins. It plays a stimulatory role in activation of the Erk/MAP kinase pathway by receptor tyrosine kinase signaling. Mutations in this protein are a cause of Noonan syndrome as well as acute myeloid leukemia. Source: Recombinant protein corresponding to aa1-593 from human SHP-2, fused to His-Tag at C-terminal, expressed in baculovirus. AA Sequence: MTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCSAGIGRTG TFIVIDILID IIREKGVDCD IDVPKTIQMV RSQRSGMVQT EAQYRFIYMA VQHYIETLQR RIEEEQKSKR KGHEYTNIKY SLADQTSGDQ SPLPPCTPTP PCAEMREDSA RVYENVGLMQ QQKSFR Biological Activity: Specific activity is > 400unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of pnitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
