Sulfotransferase 1C4, also known as SuLT1C4, belongs to the SuLT subfamily, responsible for transferring a sulfo moiety from PAPS to phenol-containing compounds. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SuLT1C4 catalyzes the sulfonation of p-nitrophenol and N-hydroxy-2-acetylaminofluorene, but not dopamine. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 909bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCCTTACACGACATGGAGGATTTTACATTTGATGGAACAAAGCGCTTAAGTGTCAACTACGTGAAGGGAATTCTTCAACCGACAGACACCTGTGACATCTGGGATAAGATCTGGAACTTCCAAGCCAAGCCTGATGACCTGCTTATTTCTACCTATCCTAAAGCAGGAACAACATGGACTCAGGAGATAGTGGAATTAATACAAAATGAAGGTGATGTGGAGAAAAGTAAACGGGCACCGACTCATCAACGATTTCCTTTCCTCGAAATGAAAATCCCATCCTTAGGATCTGGTTTGGAACAAGCTCATGCAATGCCCTCACCACGGATCCTGAAAACACATCTTCCCTTTCACTTGCTGCCACCATCCTTGCTAGAGAAAAACTGTAAGATAATCTATGTAGCAAGAAATCCCAAGGACAACATGGTGTCCTATTACCATTTCCAAAGAATGAATAAAGCTCTTCCTGCTCCAGGAACATGGGAAGAGTATTTTGAGACTTTTCTGGCTGGGAAAGTGTGCTGGGGCTCCTGGCATGAACATGTGAAAGGATGGTGGGAAGCCAAAGACAAACACCGTATTCTCTATCTCTTCTATGAGGACATGAAGAAGAACCCAAAGCATGAAATTCAGAAGCTGGCAGAATTTATTGGGAAGAAATTAGATGACAAAGTTCTAGATAAAATTGTCCATTACACTTCGTTTGATGTCATGAAACAGAATCCAATGGCAAACTATTCATCGATTCCTGCTGAAATCATGGACCACTCCATTTCTCCATTCATGAGAAAAGGGGCAGTGGGAGACTGGAAGAAACACTTCACCGTGGCTCAGAATGAGAGATTTGATGAAGATTACAAGAAGAAAATGACTGATACCAGACTAACTTTCCACTTCCAGTTCTAG Translation Sequence: MALHDMEDFT FDGTKRLSVN YVKGILQPTD TCDIWDKIWN FQAKPDDLLI STYPKAGTTWTQEIVELIQN EGDVEKSKRA PTHQRFPFLE MKIPSLGSGL EQAHAMPSPR ILKTHLPFHLLPPSLLEKNC KIIYVARNPK DNMVSYYHFQ RMNKALPAPG TWEEYFETFL AGKVCWGSWHEHVKGWWEAK DKHRILYLFY EDMKKNPKHE IQKLAEFIGK KLDDKVLDKI VHYTSFDVMKQNPMANYSSI PAEIMDHSIS PFMRKGAVGD WKKHFTVAQN ERFDEDYKKK MTDTRLTFHFQF Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
