Transcriptional adapter 3 isoform a, also known as TATD3, is a subunit of 2 histone acetyltransferase complexes. TATD3 is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. Alternate splicing results in multiple transcript variants of TADA3. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1299bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAGTGAGTTGAAAGACTGCCCCTTGCAGTTCCACGACTTCAAGTCTGTGGATCACCTGAAGGTCTGTCCCCGCTACACGGCAGTGCTGGCACGCTCTGAGGATGATGGCATCGGCATCGAGGAGCTGGACACCCTGCAGCTGGAGCTGGAGACCCTGCTGTCTTCTGCCAGCCGGCGCCTGCGTGTGCTTGAGGCCGAAACCCAGATCCTCACCGACTGGCAGGATAAGAAAGGTGACAGACGATTCCTGAAGCTGGGTCGAGACCATGAACTTGGAGCTCCCCCCAAACATGGGAAGCCCAAGAAGCAGAAACTGGAAGGGAAGGCAGGACATGGGCCGGGCCCTGGCCCAGGACGGCCCAAATCCAAAAACCTTCAGCCCAAGATCCAGGAATATGAATTCACTGATGACCCTATCGACGTGCCACGGATCCCCAAAAATGATGCCCCCAACAGGTTCTGGGCTTCAGTGGAGCCCTACTGTGCTGACATCACCAGCGAGGAGGTCCGCACACTTGAGGAGTTACTGAAGCCCCCAGAAGATGAGGCTGAGCATTACAAGATCCCACCCCTGGGGAAGCACTACTCCCAGCGCTGGGCCCAGGAGGACCTGCTGGAGGAGCAGAAGGATGGGGCCCGGGCAGCGGCTGTGGCTGACAAGAAGAAAGGCCTCATGGGGCCACTGACCGAACTGGACACTAAAGATGTGGATGCCCTGCTGAAGAAGTCTGAGGCCCAGCATGAACAGCCGGAAGATGGATGCCCCTTTGGTGCCCTGACGCAGCGCCTCCTGCAGGCCCTGGTGGAGGAAAATATTATTTCCCCTATGGAGGATTCTCCTATTCCTGACATGTCTGGGAAAGAATCAGGGGCTGACGGGGCAAGCACCTCCCCTCGCAATCAGAACAAGCCCTTCAGTGTGCCGCATACTAAGTCCCTGGAGAGCCGCATCAAGGAGGAGCTAATTGCCCAGGGCCTTTTGGAGTCTGAGGACCGCCCCGCAGAGGACTCCGAGGATGAGGTCCTTGCTGAGCTTCGCAAACGGCAGGCTGAGCTGAAGGCACTTAGTGCCCACAACCGCACCAAGAAGCACGACCTGCTGAGGCTGGCAAAGGAGGAGGTGAGCCGGCAGGAGCTGAGGCAGCGGGTGCGCATGGCTGACAACGAGGTCATGGACGCCTTTCGCAAGATCATGGCTGCCCGGCAGAAGAAGCGGACTCCCACCAAGAAAGAAAAGGACCAGGCCTGGAAGACTCTGAAGGAGCGTGAGAGCATCCTGAAGCTGCTGGATGGGTAG Translation Sequence: MSELKDCPLQ FHDFKSVDHL KVCPRYTAVL ARSEDDGIGI EELDTLQLEL ETLLSSASRRLRVLEAETQI LTDWQDKKGD RRFLKLGRDH ELGAPPKHGK PKKQKLEGKA GHGPGPGPGRPKSKNLQPKI QEYEFTDDPI DVPRIPKNDA PNRFWASVEP YCADITSEEV RTLEELLKPPEDEAEHYKIP PLGKHYSQRW AQEDLLEEQK DGARAAAVAD KKKGLMGPLT ELDTKDVDALLKKSEAQHEQ PEDGCPFGAL TQRLLQALVE ENIISPMEDS PIPDMSGKES GADGASTSPRNQNKPFSVPH TKSLESRIKE ELIAQGLLES EDRPAEDSED EVLAELRKRQ AELKALSAHNRTKKHDLLRL AKEEVSRQEL RQRVRMADNE VMDAFRKIMA ARQKKRTPTK KEKDQAWKTLKERESILKLL DG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
