TCEA2 is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for TCEA2 Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 900bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGATGGGCAAGGAAGAGGAGATTGCGCGGATCGCCCGGAGGCTGGACAAGATGGTGACCAAGAAGAGCGCGGAGGGAGCCATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACGCTGCACCTGCTCCAGTCCACCCGAGTCGGGATGTCTGTCAACGCCCTTCGGAAGCAGAGCTCGGATGAGGAGGTCATTGCACTGGCCAAGTCTCTCATCAAGTCCTGGAAGAAGCTCCTGGATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCATGCCTCTGCCCACGTCCTCGAGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGAGCTGCCCAGGGCACCGTCGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAACAAGTGCCGCGAGATGCTGACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGTGCGGACTGCGAGCGCCTGTCGGCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAACACAGACATGAAGTATAAGAACCGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAGAACCCTGACCTGCGGCGGAATGTGCTGTGTGGGGCCATAACACCCCAGCAGATCGCTGTGATGACCTCAGAGGAGATGGCCAGTGATGAGCTGAAGGAGATCCGTAAGGCCATGACCAAGGAGGCCATCCGAGAGCACCAGATGGCCCGCACTGGCGGCACGCAGACAGACCTGTTCACCTGCGGCAAGTGCAGGAAAAAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGATGAGCCCATGACCACCTTTGTTGTCTGCAACGAGTGTGGAAACCGCTGGAAGTTCTGCTGA Translation Sequence: MMGKEEEIAR IARRLDKMVT KKSAEGAMDL LRELKAMPIT LHLLQSTRVG MSVNALRKQSSDEEVIALAK SLIKSWKKLL DASDAKARER GRGMPLPTSS RDASEAPDPS RKRPELPRAPSTPRITTFPP VPVTCDAVRN KCREMLTAAL QTDHDHVAIG ADCERLSAQI EECIFRDVGNTDMKYKNRVR SRISNLKDAK NPDLRRNVLC GAITPQQIAV MTSEEMASDE LKEIRKAMTKEAIREHQMAR TGGTQTDLFT CGKCRKKNCT YTQVQTRSSD EPMTTFVVCN ECGNRWKFC Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
