TDP2 is a member of a superfamily of divalent cation-dependent phosphodiesterases. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1089bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGAGTTGGGGAGTTGCCTGGAGGGCGGGAGGGAGGCGGCGGAGGAAGAGGGCGAGCCTGAGGTGAAAAAGCGGCGACTTCTGTGTGTGGAGTTTGCCTCGGTCGCAAGCTGCGATGCCGCAGTGGCTCAGTGCTTCCTGGCCGAGAACGACTGGGAGATGGAAAGGGCTCTGAACTCCTACTTCGAGCCTCCGGTGGAGGAGAGCGCCTTGGAACGCCGACCTGAAACCATCTCTGAGCCCAAGACCTATGTTGACCTAACCAATGAAGAAACAACTGATTCCACCACTTCTAAAATCAGCCCATCTGAAGATACTCAGCAAGAAAATGGCAGCATGTTCTCTCTCATTACCTGGAATATTGATGGATTAGATCTAAACAATCTGTCAGAGAGGGCTCGAGGGGTGTGTTCCTACTTAGCTTTGTACAGCCCAGATGTGATATTTCTACAGGAAGTTATTCCCCCATATTATAGCTACCTAAAGAAGAGATCAAGTAATTATGAGATTATTACAGGTCATGAAGAAGGATATTTCACAGCTATAATGTTGAAGAAATCAAGAGTGAAATTAAAAAGCCAAGAGATTATTCCTTTTCCAAGTACCAAAATGATGAGAAACCTTTTATGTGTGCATGTGAACGTGTCAGGAAATGAGCTTTGCCTTATGACATCCCATTTGGAGAGCACCAGAGGGCATGCTGCGGAACGAATGAATCAGTTAAAAATGGTTTTAAAGAAAATGCAAGAGGCTCCAGAGTCAGCTACAGTTATATTTGCAGGAGATACAAATCTAAGGGATCGAGAGGTTACCAGATGTGGTGGTTTACCCAACAACATTGTGGATGTCTGGGAGTTTTTGGGCAAACCTAAACATTGCCAGTATACATGGGATACACAAATGAACTCTAATCTTGGAATAACTGCTGCTTGTAAACTTCGTTTTGATCGAATATTTTTCAGAGCAGCAGCAGAAGAGGGACACATTATTCCCCGAAGTTTGGACCTTCTTGGATTAGAAAAACTGGACTGTGGTAGATTTCCTAGTGATCACTGGGGTCTTCTGTGCAACTTAGATATAATATTGTAA Translation Sequence: MELGSCLEGG REAAEEEGEP EVKKRRLLCV EFASVASCDA AVAQCFLAEN DWEMERALNSYFEPPVEESA LERRPETISE PKTYVDLTNE ETTDSTTSKI SPSEDTQQEN GSMFSLITWNIDGLDLNNLS ERARGVCSYL ALYSPDVIFL QEVIPPYYSY LKKRSSNYEI ITGHEEGYFTAIMLKKSRVK LKSQEIIPFP STKMMRNLLC VHVNVSGNEL CLMTSHLEST RGHAAERMNQLKMVLKKMQE APESATVIFA GDTNLRDREV TRCGGLPNNI VDVWEFLGKP KHCQYTWDTQMNSNLGITAA CKLRFDRIFF RAAAEEGHII PRSLDLLGLE KLDCGRFPSD HWGLLCNLDIIL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
