TYW5 (TRNA-YW Synthesizing Protein 5) acts as a component of the wybutosine biosynthesis pathway. It catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl) wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 939bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCCGGGCAGCACCTCCCGGTACCCCGGCTGGAGGGCGTTTCTCGGGAGCAGTTCATGCAGCACCTCTACCCACAGAGAAAACCTCTTGTGTTGGAAGGGATTGATTTGGGGCCATGTACAAGCAAATGGACAGTGGATTACCTAAGCCAAGTTGGAGGGAAGAAAGAAGTAAAGATTCATGTTGCTGCAGTTGCACAGATGGACTTCATTAGTAAGAACTTTGTATATAGAACTTTACCTTTTGACCAGTTGGTCCAGAGGGCAGCTGAAGAGAAACATAAAGAATTCTTTGTTTCAGAGGATGAGAAATACTACTTACGGTCACTTGGAGAAGACCCTAGAAAGGATGTTGCAGATATCAGAAAGCAGTTTCCTTTGTTGAAAGGAGATATTAAGTTTCCAGAATTCTTCAAAGAGGAACAGTTCTTTTCCAGTGTTTTTCGAATTAGTTCACCAGGATTACAACTATGGACTCATTATGATGTAATGGATAATTTGTTAATACAAGTGACAGGAAAAAAGCGTGTTGTACTCTTCAGTCCTCGAGATGCCCAGTATTTATATTTAAAAGGTACTAAATCAGAAGTACTGAATATAGATAACCCAGACTTGGCTAAATATCCACTTTTTTCCAAGGCTAGAAGATATGAATGTTCCCTTGAAGCTGGTGATGTATTATTCATTCCTGCTTTATGGTTCCATAATGTAATTTCTGAAGAGTTTGGAGTGGGAGTGAATATCTTTTGGAAGCACCTTCCATCTGAATGCTATGATAAAACAGATACCTATGGAAACAAAGATCCTACAGCAGCATCAAGAGCTGCACAAATTCTGGACAGAGCCTTGAAAACACTGGCCGAGTTACCAGAGGAATATAGGGACTTCTATGCACGACGAATGGTCCTACACATTCAAGACAAAGCCTACAGCAAGAACTCTGAGTAA Translation Sequence: MAGQHLPVPR LEGVSREQFM QHLYPQRKPL VLEGIDLGPC TSKWTVDYLS QVGGKKEVKI HVAAVAQMDF ISKNFVYRTL PFDQLVQRAA EEKHKEFFVS EDEKYYLRSL GEDPRKDVAD IRKQFPLLKG DIKFPEFFKE EQFFSSVFRI SSPGLQLWTH YDVMDNLLIQ VTGKKRVVLF SPRDAQYLYL KGTKSEVLNI DNPDLAKYPL FSKARRYECS LEAGDVLFIP ALWFHNVISE EFGVGVNIFW KHLPSECYDK TDTYGNKDPT AASRAAQILD RALKTLAELP EEYRDFYARR MVLHIQDKAY SKNSE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
