Biomatrix Coated Plates (White and Clear) (Liver)

Produktübersicht

• Artikelname: Biomatrix Coated Plates (White and Clear) (Liver)
• Artikelnummer: USB-553707
• Hersteller Artikelnummer: 553707
• Alternativnummer: USB-553707-5X96
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Liver Biomatrix is a novel matrix isolated from decellularized liver tissue. This proprietary process retains the liver matrix biochemistry leaving the matrix scaffold intact. Following the decellulariziation process, the matrix scaffold is reduced to um sized particles in suspension which is coated onto multi-well plates and dishes. Biomatrix coated plates and dishes provide the ultimate microenvironment for maintaining functional hepatocytes in culture longer than on Collagen I. In addition, stem cells committed to the endodermal lineage differentiate into functional hepatocytes within a few days maintaining function for greater than 4 weeks in culture. Preparing Liver Biomatrix Coated Plates and Dishes For Receiving Primary Hepatocytes: 1. Prepare Hepatocyte Maintenance media according to product insert 2. Remove the biomatrix coated plate from the package aseptically 3. For best results, rehydrate the biomatrix overnight. If there are time constraints, minimal rehydration time is at least 2 hours prior to use. Caution: When adding liquid media to the well or dish, slowly disperse the media down the side of the well or dish. DO NOT deliver liquid media directly onto the biomatrix coating as this could force some biomatrix particles to release from the plate leaving a hole in the biomatrix layer. 4. Add Hepatocyte Maintenance Medium to each well or plate and place in humidified incubator. Refer to Table 1 for volume. 5. When ready to add cells, remove the rehydration media, wash once with Hepatocyte Maintenance Medium taking care not to dispense media directly onto the biomatrix coating. Caution: When aspirating liquid from the biomatrix coated plate/well or dish, slowly lower the aspiration pipette tip to the media surface taking care not to touch the bottom of the biomatrix coating on plate/well or dish. DO NOT touch the biomatrix with aspiration pipette tip as this will result in aspiration of biomatrix particles that are in direct contact to the tip. 6. Add cell suspension in Hepatocyte Maintenance Media. Refer to Table 1 for volume and plating densities. Note: Ensure that the cell suspension is homogenous when adding to the biomatrix coated plate. The cells will settle onto the biomatrix coating within 10 minutes so after adding the cells let the plates or dishes set for 10 minutes before moving them into the incubator. Preparing Liver Biomatrix Coated Plates and Dishes For Receiving Hepatoblasts or Stem Cells: 1. Prepare Kubotas StemCell Growth Media for stem cells of endodermal lineage or Kubotas Hepatoblast Growth Media for lineage committed hepatoblasts according to product inserts. 2. Remove the biomatrix coated plate or dish from the package aseptically and place in safety cabinet. 3. For best results, rehydrate the biomatrix overnight. If there are time constraints, minimal rehydration time is at least 2 hours prior to use. Caution: When adding liquid media to the well or dish, slowly disperse the media down the side of the well. DO NOT deliver liquid media directly onto the biomatrix coating as this could force some biomatrix particles to release from the plate leaving a hole in the biomatrix layer. 4. Add Kubotas StemCell or Hepatoblast Growth Media to each well and place plate in humidified incubator. Refer to Table 1 for volume. 5. When ready to add cells, remove the rehydration media, wash once with Kubotas StemCell or Hepatoblast Growth Media taking care not to dispense media directly onto the biomatrix coating. Caution: When aspirating liquid from the biomatrix coated plate/well or dish, slowly lower the aspiration pipette tip to the media surface taking care not to touch the biomatrix coating. DO NOT touch the biomatrix with aspiration pipette tip as this will result in aspiration of biomatrix particles that are in direct contact to the tip. 6. Add cell suspension in Kubotas StemCell or Hepatoblast Growth Media. Refer to Table 1 for volume and plating densities. Note: Ensure that the cell suspension