Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5 UTRs. [provided by RefSeq] Source: Recombinant protein corresponding to IARS, Recombinant, Human, Met1~Arg500, His Tag, N-terminal Sequencxe: See Image Library Entrez GeneID: 3376 Gene Symbol: IARS Gene Alias: FLJ20736, IARS1, ILRS, PRO0785 Subcellular Location: Cytoplasm Predicted Molecular Mass: 61.7kD Accurate Molecular Mass: 62kD Residues & Tags: Met1~Arg500 with N-terminal His Tag Applications: Suitable for use in Positive Control, Immunogen, SDS-PAGE, WB. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Host: E. coli Theoretical MW (kD): 35.75 Isoelectric Point: 5.6 Quality Control: 12.5% SDS-PAGE Stained with Coomassie Blue. Endotoxin: 1.0EU per 1ug (LAL) Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70C. Aliquots are stable for 3-6 months after receipt at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Lyophilized powder in 20mM Tris, 150mM NaCl, pH8.0, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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