5-methylthioadenosine/S-adenosylhomocysteine Nucleosidase, Recombinant, E. coli O9:H4, aa1-232, His-Tag, Myc-Tag

Produktübersicht

• Artikelname: 5-methylthioadenosine/S-adenosylhomocysteine Nucleosidase, Recombinant, E. coli O9:H4, aa1-232, His-Tag, Myc-Tag
• Artikelnummer: USB-583442
• Hersteller Artikelnummer: 583442
• Alternativnummer: USB-583442-20, USB-583442-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Catalyzes the irreversible cleavage of the glycosidic bond in both 5-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5-deoxyadenosine accumulation. Source: Recombinant protein corresponding to aa1-232 from Escherichia coli O9:H4 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~31.8kD Amino Acid Sequence: MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVALLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADVTAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSSLMVESLVQKLAHG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 31.8
• UniProt: A7ZWA7
• Reinheit: 85% (SDS-PAGE)
• Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.