DET1- and DDB1-associated Protein 1, Recombinant, Human, aa2-102, His-Tag
Artikelnummer:
USB-584234
Hersteller Artikelnummer:
584234
Alternativnummer:
USB-584234-20,USB-584234-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex. Recombinant protein corresponding to aa2-102 from human DET1- and DDB1-associated protein 1, fused to 10X His-Tag at N-terminal, expressed in E.coli. Swiss/Uniprot: Q9BW61 Molecular Weight: ~17.7kD Amino Acid Sequence: ADFLKGLPVYNKSNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKNAAKKRDQEQVELEGESSAPPRKVARTDSPDMHEDT Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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