DNA-binding Protein HMf-2, Recombinant, Methanothermus fervidus, aa1-69, His-Tag
Artikelnummer:
USB-584287
Hersteller Artikelnummer:
584287
Alternativnummer:
USB-584287-20,USB-584287-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Binds and compacts DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation in vitro. Source: Recombinant protein corresponding to aa1-69 from Methanothermus fervidus DNA-binding protein HMf-2, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~11.7kD Amino Acid Sequence: MELPIAPIGRIIKDAGAERVSDDARITLAKILEEMGRDIASEAIKLARHAGRKTIKAEDIELAVRRFKK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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