ESAT-6-like Protein EsxB, Recombinant, Mycobacterium tuberculosis, aa2-100, His-Tag
Artikelnummer:
USB-584463
Hersteller Artikelnummer:
584463
Alternativnummer:
USB-584463-20,USB-584463-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA. Source: Recombinant protein corresponding to aa2-100 from Mycobacterium tuberculosis ESAT-6-like protein EsxB, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~14.7kD Amino Acid Sequence: AEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAGVQYSRADEEQQQALSSQMGF Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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