Glucose-6-phosphate 1-dehydrogenase, Recombinant, E. coli, aa1-486, GST-Tag

Produktübersicht

• Artikelname: Glucose-6-phosphate 1-dehydrogenase, Recombinant, E. coli, aa1-486, GST-Tag
• Artikelnummer: USB-584677
• Hersteller Artikelnummer: 584677
• Alternativnummer: USB-584677-20,USB-584677-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone., Probable source of extracellular death factor (EDF, sequence Asn-Asn-Trp-Asn-Asn, NNWNN) following processing and amidation. This pentapeptide stimulates cell death mediated by MazF. Artificial peptides with altered sequence show that NNGNN, GNWNG and NWN no longer stimulate MazFs endoribonuclease activity, other peptides (NNGN, GNWMM, NNWNG, NNNWNNN) retain MazF-stimulating activity. NNWNN, NNGN, GNWMM and NNWNG prevent cognate antitoxin MazE from inhibiting MazF, although NNNWNNN stimulates MazF it does not do so in the presence of MazE. EDF also stimulates ChpBs endoribonuclease activity in vitro, in this case NWN partially stimulates ChpB, whereas NNGNN, GNWNN, NNWNG, GNWNG and NNNWNNN do not. Only the wild-type EDF peptide prevents cognate antitoxin ChpS from inhibiting ChpB. Source: Recombinant protein corresponding to aa1-486 from Escherichia coli Glucose-6-phosphate 1-dehydrogenase, fused to GST-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~82.0kD Amino Acid Sequence: MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEINDQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 82
• UniProt: P0AC53
• Reinheit: 90% (SDS-PAGE)
• Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.