Provides the (R)-glutamate required for cell wall biosynthesis. Source: Recombinant protein corresponding to aa1-255 from Helicobacter pylori Glutamate racemase, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~35.9kD Amino Acid Sequence: MKIGVFDSGVGGFSVLKSLLKAQLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFFKPHQIKLLIVACNTASALALEEMQKHSKIPIVGVIEPSILAIKQQVKEKNAPILVLGTKATIQSNAYDNALKQQGYLNVSHLATSLFVPLIEESILEGELLETCMRYYFTPLKILPEVIILGCTHFPLIAQKIEGYFMEHFALSTPPLLIHSGDAIVEYLQQKYALKKNAHAFPKVEFHASGDVIWLEKQAKEWLKL Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
