Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue (By similarity). Source: Recombinant protein corresponding to aa36-362 from mouse Glutaminyl-peptide cyclotransferase, fused to His-Tag at N-terminal, expressed in E.coli. Amino Acid Sequence: AWTQE KNHHQPAHLN SSSLQQVAEG TSISEMWQND LRPLLIERYP GSPGSYSARQ HIMQRIQRLQ AEWVVEVDTF LSRTPYGYRS FSNIISTLNP EAKRHLVLAC HYDSKYFPRW DSRVFVGATD SAVPCAMMLE LARALDKKLH SLKDVSGSKP DLSLRLIFFD GEEAFHHWSP QDSLYGSRHL AQKMASSPHP PGSRGTNQLD GMDLLVLLDL IGAANPTFPN FFPKTTRWFN RLQAIEKELY ELGLLKDHSL ERKYFQNFGY GNIIQDDHIP FLRKGVPVLH LIASPFPEVW HTMDDNEENL HASTIDNLNK IIQVFVLEYL HL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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