Matrix M2-1, Recombinant, Human respiratory syncytial virus B, aa1-195, His-Tag
Artikelnummer:
USB-585334
Hersteller Artikelnummer:
585334
Alternativnummer:
USB-585334-20,USB-585334-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Acts as a tetrameric transcription processivity factor that binds in a competitive manner to RNA and the phosphoprotein (P) to prevent premature termination during transcription. Transcription anti-terminator that enhances readthrough of intergenic junctions during viral transcription. Preferentially binds to poly(A)-rich sequences. Plays a role in the association of the matrix protein with the nucleocapsid, which initiates assembly and budding. Also, can activate host NF-kappa-B through association with host RELA. Source: Recombinant protein corresponding to aa1-195 from human respiratory syncytial virus B Matrix M2-1, fused to 6X His-Tag at N-terminal and 6X His-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~25.5kD Amino Acid Sequence: MSRRNPCKFEIRGHCLNGRRCHYSHNYFEWPPHALLVRQNFMLNKILKSMDKSIDTLSEISGAAELDRTEEYALGIVGVLESYIGSINNITKQSACVAMSKLLIEINSDDIKKLRDNEEPNSPKIRVYNTVISYIESNRKNNKQTIHLLKRLPADVLKKTIKNTLDIHKSIIISNPKESTVNDQNDQTKNNDITG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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