Myosin-9, Recombinant, Rat, aa2-241, His-Tag, Myc-Tag

Artikelnummer: USB-585460
Artikelname: Myosin-9, Recombinant, Rat, aa2-241, His-Tag, Myc-Tag
Artikelnummer: USB-585460
Hersteller Artikelnummer: 585460
Alternativnummer: USB-585460-20,USB-585460-100
Hersteller: US Biological
Kategorie: Molekularbiologie
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis. Promotes cell motility in conjunction with S100A4. During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction, this function is mechanically antagonized by MYH10. Source: Recombinant protein corresponding to aa2-241 from rat Myosin-9, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~34.6kD Amino Acid Sequence: AQQAADKYLYVDKNFINNPLAQADCGAKKLVWVPSTKNGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVDMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht: 34.6
UniProt: Q62812
Reinheit: 85% (SDS-PAGE)
Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.