Functions as an intestinal M-cells transcytotic receptor specific of type-I-piliated bacteria that participate in the mucosal immune response toward these bacteria. At the apical membrane of M-cells binds fimH, a protein of the bacteria type I pilus tip. Internalizes bound bacteria, like E.coli and S.typhimurium, from the lumen of the intestine and delivers them, through M-cells, to the underlying organized lymphoid follicles where they are captured by antigen-presenting dendritic cells to ellicit a mucosal immune response. Source: Recombinant protein corresponding to aa22-531 from mouse Pancreatic secretory granule membrane major glycoprotein GP2, fused to 6X His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~59.0kD Amino Acid Sequence: STIHQGYGRPRNSSNLDLDCGSPDSPSSGICFDPCQNHTVLNDPTRSTENNDSSVAWCDDNLHGWYRFVGDGGVKMPETCVSVFRCHTSAPMWLSGSHPILGDGIVSHTACANWNENCCFWRSEVQVKACSEELGEYHVYKLQGTPECSLRYCTDPSTAPKNCEITCRPEEECVFQNNNWSCVCRQDLHVSDSQSLQPLLDCGDNEIKVKLDKCLLGGMGFKEEIIAYLNDRNCNGTMQDEPNNWVSMTSPVVANYCGNILEKNGTHAIYRNTLSLATDFIIRDFRVNVNFQCAYPLDMSVSLETALQPIVSSLTVDVDGAGEFNVKMALFQDQSYTNPYEGAEVLLPVESILYVGVLLNRGDTSRFKLLLTNCYATPSEDRHDPVKYFIIKNRCPNQRDSTINVRENGVSSESRFSVQMFMFAGNYDLVFLHCEVYLCDSTTEQCQPSCSTNRLRSSRPAIDYNRVLDLGPITKRSAQSSATSKGTPHTTGFLLAWPMFFLPVFLALLF Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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