Replication Protein E1, Recombinant, Human papillomavirus type 11, aa452-602, His-Tag

Produktübersicht

• Artikelname: Replication Protein E1, Recombinant, Human papillomavirus type 11, aa452-602, His-Tag
• Artikelnummer: USB-586152
• Hersteller Artikelnummer: 586152
• Alternativnummer: USB-586152-20,USB-586152-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication. Source: Recombinant protein corresponding to aa452-602 from human papillomavirus type 11 Replication protein E1, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~23.1kD Amino Acid Sequence: IEFIPFLSKLKLWLHGTPKKNCIAIVGPPDTGKSCFCMSLIKFLGGTVISYVNSCSHFWLQPLTDAKVALLDDATQPCWTYMDTYMRNLLDGNPMSIDRKHRALTLIKCPPLLVTSNIDISKEEKYKYLHSRVTTFTFPNPFPFDRNGNAV Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 23.1
• UniProt: P04014
• Reinheit: 85% (SDS-PAGE)
• Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.