GTP-dependent RNA ligase that is involved in tRNA splicing and RNA repair. Joins RNA with 2,3-cyclic-phosphate or 3-phosphate ends to RNA with 5-hydroxy ends. Also acts as a DNA ligase in case of DNA damage by splicing dirty DNA breaks, characterized by 3-phosphate (or cyclic-phosphate) and 5-hydroxy ends that cannot be sealed by classical DNA ligases. Source: Recombinant protein corresponding to aa1-408 from Escherichia coli RNA-splicing ligase RtcB, fused to 6X His-SUMO-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~61.2kD Amino Acid Sequence: MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQAIETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVVCVKG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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