Hydrolase that can remove Lys-48-linked conjugated ubiquitin from proteins. Can also bind to polyubiquitin chains of different linkage types, including Lys-6, Lys-11, Lys-29, Lys-33 and Lys-63. May play a regulatory role at the level of protein turnover. Source: Recombinant protein corresponding to aa1-601 from mouse Ubiquitin carboxyl-terminal hydrolase MINDY-2, fused to GST-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~93.1kD Amino Acid Sequence: MENSPDSPQPLELGVAAGRVSPPEGRRRGGREAEDGPAGRAVDSGGQGAAAAAARSSLGDPTSPSQLGCGAGSDLKDGASSSPAASEVPSRGQHKVTASPELAEAAAGRGSGPVGDTGTCRVEQAAEEPSSTGAPSSSCSEPSPPGDSPSLDSLESFSNLHSFPSSSEFNSEEGAETRVPEDVEEGAAGPPRAAPLCKEEEEDPAQVLAASKERFPGQSVYHIKWIQWKEENTPIITQNENGPCPLLAILNVLLLAWKVKLPPMMEIITAEQLMEYLGDYMLEAKPKEISEIQRVNYEQNMSDAMAILHKLQTGLDVNVRFTGVRVFEYTPECIVFDLLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSQLVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKIVWESLHNVDGDGNFCDSEFHLRPPSDPETVYKGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKLQEEEDRRASQYYQEQEQAQAVVTTTTPSTQAQQGQPAQASPSSIKQPGNSERKRKEPREKDKEKEKEKNSCVIL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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