Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis Partial Recombinant protein corresponding to aa132-288 from human Fibroblast growth factor 2, expressed in E.coli. Molecular Weight: ~17.4kD Biological Activity: The ED50 as determined in a cell proliferation assay using BALB/c 3T3 cells is less than 10ng/ml. Amino Acid Sequence: GTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 0.2um filtered 20mM Tris-HCl, pH 7.5, 150mM sodum chloride. Reconstitute with sterile ddH2O, to a concentration of 0.1-1mg/ml.
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