| The Matrix Metalloproteinase 2 (MMP2) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP2 in bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 1.56-100ng/ml Sensitivity: 0.65ng/ml Precision: Intra-Assay %CV: <10% Inter-Assay %CV: <12% Kit Components: *589507A: Microtiter Strips, 1x96 wells Pre-coated, ready to use *589507B: Standard, 2x1 vial 589507C: Standard Diluent, 20ml *589507D: Detection Reagent A (green), 1x120ul *589507E: Detection Reagent B (red), 1x120ul 589507F: Assay Diluent A, 1x12ml 589507G: Assay Diluent B, 1x12ml 589507H: TMB Substrate, 1x9ml *589507J: Positive Control, Lyophilized, xxng/ml, 1x1vial 589507K: Stop Solution, 1x6ml 589507L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (589507K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *589507A, *589507B, *589507D, *589507E and *589507J at -20C. After reconstitution of *589507J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substmousee. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately. |
