| The Myosin Heavy Chain 1 (MYH1) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MYH1 in bovine tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.059ng/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *589540A: Microtiter Strips, 1x96 wells Pre-coated, ready to use *589540B: Standard, 2x1 vial 589540C: Standard Diluent, 20ml *589540D: Detection Reagent A (green), 1x120ul *589540E: Detection Reagent B (red), 1x120ul 589540F: Assay Diluent A, 1x12ml 589540G: Assay Diluent B, 1x12ml 589540H: TMB Substrate, 1x9ml *589540J: Positive Control, Lyophilized, 1.92ng/ml, 1x1vial 589540K: Stop Solution, 1x6ml 589540L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (589540K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *589540A, *589540B, *589540D, *589540E and *589540J at -20C. After reconstitution of *589540J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 589540J: Positive Control: Intended Use: The Positive Control is from a bovine liver tissue homogenates sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The positive control vial contains bovine MYH1 in bovine liver tissue homogenates (lyophilized powder). Note: The bovine based serum used in this product are from healthy bovine of SPF standard (specific pathogen-free animals). Expected Values: 1.92ng/ml Usage: Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substmousee. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately. |
