Acetylcholine Assay Kit, BioAssay(TM)

Produktübersicht

• Artikelname: Acetylcholine Assay Kit, BioAssay(TM)
• Artikelnummer: USB-A0526-32A
• Hersteller Artikelnummer: A0526-32A
• Alternativnummer: USB-A0526-32A-1
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Acetylcholine Assay Kit provides a simple, direct and high throughput assay for measuring acetylcholine in biological samples. Principle of the Assay: In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585nm) is directly proportional to the acetylcholine concentration in the sample. Applications: Assays: Determination of acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue and cell culture. Drug Discovery/Pharmacology: Investigation of effects of drugs on acetylcholine metabolism. Sample Size: 20ul Linear Detection Range: 100-200uM (fluorometric assay), 0.4-10uM (colorimetric assay) Kit Components: A0526-32A1: ACHE Enzyme, 1x120ul A0526-32A2: Enzyme Mix, 1x120ul A0526-32A3: Acetylcholine Standard (2mM), 1x400ul A0526-32A4: Dye Reagent, 1x120ul A0526-32A5: Assay Buffer, 1x10ml Storage and Stability: Store all components at -20C. Kit is stable for up to 3 months upon receipt.